Bioassay Guided Isolation of Anti-Inflammatory Compounds from Bauhinia variegata L.: A Key Ingredient in Herbo-Mineral Formulation, Gandmala Kandan Ras

Medicinal herb Bauhinia variegata L. is the main ingredient in “Gandmala Kandan Ras” herbomineral medicine used Ayurveda for treatment of swelling, inflammation and tumors. This study aimed to isolate anti-inflammatory compounds from methanolic extract aerial parts variegata. Through bioassay-guided isolation, three known flavonoids, namely kaempferol, ombuin quercetin were identified variega. The primary screening by protein denaturation method revealed a significant percentage inhibition kaempferol quercetin. assays against COX-1/2 enzymes showed activity these two compounds, compared standard drug, indomethacin. Of which, as potent non-selective inhibitor COX1 COX2 with minimal inhibitory concentration values 172.05±4.29 220.62±9.13 nM, respectively; while selective IC50 154.86±5.60 nM. results provided evidence that supports Ayurvedic usage Gandmala Ras formulation inflammation, which was attributed natural active indicated plant could be considered an excellent source remedial inflammation.